diff --git a/XP.Camera/Calibration/ICalibrationCaptureService.cs b/XP.Camera/Calibration/ICalibrationCaptureService.cs
new file mode 100644
index 0000000..c60ca15
--- /dev/null
+++ b/XP.Camera/Calibration/ICalibrationCaptureService.cs
@@ -0,0 +1,73 @@
+using System.Drawing;
+using System.Windows.Media.Imaging;
+
+namespace XP.Camera.Calibration;
+
+/// <summary>
+/// 标定采集服务接口
+/// 提供"一键采集"能力：读取编码器坐标 + 拍图 + 识别标记中心
+/// </summary>
+public interface ICalibrationCaptureService
+{
+    /// <summary>是否可用（相机已连接、运动系统就绪）</summary>
+    bool IsAvailable { get; }
+
+    /// <summary>
+    /// 采集当前标定点
+    /// </summary>
+    /// <returns>采集结果，失败时返回 null</returns>
+    CaptureResult? CaptureCurrentPoint();
+
+    /// <summary>
+    /// 获取当前导航相机图像
+    /// </summary>
+    BitmapSource? CaptureImage();
+
+    /// <summary>
+    /// 启动实时预览（将相机实时画面推送到 LiveImageUpdated 事件）
+    /// </summary>
+    void StartLivePreview();
+
+    /// <summary>
+    /// 停止实时预览
+    /// </summary>
+    void StopLivePreview();
+
+    /// <summary>
+    /// 实时画面更新事件
+    /// </summary>
+    event EventHandler<LiveImageEventArgs>? LiveImageUpdated;
+}
+
+/// <summary>
+/// 实时画面事件参数
+/// </summary>
+public class LiveImageEventArgs : EventArgs
+{
+    public BitmapSource Image { get; }
+    public LiveImageEventArgs(BitmapSource image) => Image = image;
+}
+
+/// <summary>
+/// 单次采集结果
+/// </summary>
+public class CaptureResult
+{
+    /// <summary>标记中心像素坐标 X（亚像素）</summary>
+    public double PixelX { get; set; }
+
+    /// <summary>标记中心像素坐标 Y（亚像素）</summary>
+    public double PixelY { get; set; }
+
+    /// <summary>平台编码器坐标 X (mm)</summary>
+    public double WorldX { get; set; }
+
+    /// <summary>平台编码器坐标 Y (mm)</summary>
+    public double WorldY { get; set; }
+
+    /// <summary>采集的图像</summary>
+    public BitmapSource? Image { get; set; }
+
+    /// <summary>检测到的标记轮廓点集</summary>
+    public System.Drawing.Point[]? ContourPoints { get; set; }
+}
diff --git a/XP.Camera/Calibration/ViewModels/CalibrationViewModel.cs b/XP.Camera/Calibration/ViewModels/CalibrationViewModel.cs
index 90c7acd..404b0f9 100644
--- a/XP.Camera/Calibration/ViewModels/CalibrationViewModel.cs
+++ b/XP.Camera/Calibration/ViewModels/CalibrationViewModel.cs
@@ -11,16 +11,20 @@ namespace XP.Camera.Calibration.ViewModels;
 public class CalibrationViewModel : BindableBase
 {
     private readonly ICalibrationDialogService _dialogService;
+    private readonly ICalibrationCaptureService? _captureService;
     private readonly CalibrationProcessor _calibrator = new();
     private Image<Bgr, byte>? _currentImage;
     private static readonly ILogger _logger = Log.ForContext<CalibrationViewModel>();
     private BitmapSource? _imageSource;
+    private BitmapSource? _frozenImage;
     private string _statusText = Res.CalibrationStatusReady;
     private bool _showWorldCoordinates;
+    private bool _isLiveView = true;
 
-    public CalibrationViewModel(ICalibrationDialogService dialogService)
+    public CalibrationViewModel(ICalibrationDialogService dialogService, ICalibrationCaptureService? captureService = null)
     {
         _dialogService = dialogService;
+        _captureService = captureService;
         CalibrationPoints = new ObservableCollection<CalibrationProcessor.CalibrationPoint>();
 
         LoadImageCommand = new DelegateCommand(LoadImage);
@@ -29,6 +33,16 @@ public class CalibrationViewModel : BindableBase
             .ObservesProperty(() => CalibrationPoints.Count);
         SaveCalibrationCommand = new DelegateCommand(SaveCalibration);
         LoadCalibrationCommand = new DelegateCommand(LoadCalibration);
+        CapturePointCommand = new DelegateCommand(CapturePoint, CanCapturePoint);
+        DeleteSelectedPointCommand = new DelegateCommand(DeleteSelectedPoint, () => SelectedPoint != null)
+            .ObservesProperty(() => SelectedPoint);
+
+        // 启动实时预览
+        if (_captureService != null)
+        {
+            _captureService.LiveImageUpdated += OnLiveImageUpdated;
+            _captureService.StartLivePreview();
+        }
     }
 
     public ObservableCollection<CalibrationProcessor.CalibrationPoint> CalibrationPoints { get; }
@@ -39,6 +53,14 @@ public class CalibrationViewModel : BindableBase
         set => SetProperty(ref _imageSource, value);
     }
 
+    private BitmapSource? _overlayImage;
+    /// <summary>叠加层图像（显示检测到的轮廓和中心点）</summary>
+    public BitmapSource? OverlayImage
+    {
+        get => _overlayImage;
+        set => SetProperty(ref _overlayImage, value);
+    }
+
     public string StatusText
     {
         get => _statusText;
@@ -51,11 +73,48 @@ public class CalibrationViewModel : BindableBase
         set => SetProperty(ref _showWorldCoordinates, value);
     }
 
+    public bool IsLiveView
+    {
+        get => _isLiveView;
+        set
+        {
+            if (SetProperty(ref _isLiveView, value))
+            {
+                RaisePropertyChanged(nameof(LiveViewButtonText));
+                if (value)
+                {
+                    // 切回实时：恢复实时预览
+                    _captureService?.StartLivePreview();
+                }
+                else
+                {
+                    // 切到当前：冻结当前帧
+                    _frozenImage = _imageSource;
+                    _captureService?.StopLivePreview();
+                }
+            }
+        }
+    }
+
+    public string LiveViewButtonText => _isLiveView ? "⏸ 冻结" : "▶ 实时";
+
     public DelegateCommand LoadImageCommand { get; }
     public DelegateCommand LoadCsvCommand { get; }
     public DelegateCommand CalibrateCommand { get; }
     public DelegateCommand SaveCalibrationCommand { get; }
     public DelegateCommand LoadCalibrationCommand { get; }
+    public DelegateCommand CapturePointCommand { get; }
+    public DelegateCommand DeleteSelectedPointCommand { get; }
+
+    /// <summary>是否支持采集模式（有采集服务注入）</summary>
+    public bool IsCaptureAvailable => _captureService?.IsAvailable == true;
+
+    private CalibrationProcessor.CalibrationPoint? _selectedPoint;
+    public CalibrationProcessor.CalibrationPoint? SelectedPoint
+    {
+        get => _selectedPoint;
+        set => SetProperty(ref _selectedPoint, value);
+    }
 
     private void LoadImage()
     {
@@ -130,6 +189,59 @@ public class CalibrationViewModel : BindableBase
         }
     }
 
+    private bool CanCapturePoint() => _captureService?.IsAvailable == true;
+
+    private void CapturePoint()
+    {
+        if (_captureService == null) return;
+
+        try
+        {
+            var result = _captureService.CaptureCurrentPoint();
+            if (result == null)
+            {
+                StatusText = "采集失败：未能识别标记点，请确认标记在视野内";
+                return;
+            }
+
+            CalibrationPoints.Add(new CalibrationProcessor.CalibrationPoint
+            {
+                PixelX = result.PixelX,
+                PixelY = result.PixelY,
+                WorldX = result.WorldX,
+                WorldY = result.WorldY
+            });
+
+            // 更新图像显示
+            if (result.Image != null)
+            {
+                ImageSource = result.Image;
+            }
+
+            // 绘制检测结果叠加层（轮廓 + 中心点）
+            DrawDetectionOverlay(result);
+
+            StatusText = $"已采集第 {CalibrationPoints.Count} 个点: 像素({result.PixelX:F1}, {result.PixelY:F1}) → 物理({result.WorldX:F3}, {result.WorldY:F3})";
+            _logger.Information("标定点采集: Pixel=({PixelX:F1}, {PixelY:F1}), World=({WorldX:F3}, {WorldY:F3})",
+                result.PixelX, result.PixelY, result.WorldX, result.WorldY);
+        }
+        catch (Exception ex)
+        {
+            StatusText = $"采集异常: {ex.Message}";
+            _logger.Error(ex, "标定点采集失败");
+        }
+    }
+
+    private void DeleteSelectedPoint()
+    {
+        if (SelectedPoint != null && CalibrationPoints.Contains(SelectedPoint))
+        {
+            CalibrationPoints.Remove(SelectedPoint);
+            SelectedPoint = null;
+            StatusText = $"已删除，剩余 {CalibrationPoints.Count} 个标定点";
+        }
+    }
+
     public PointF ConvertPixelToWorld(PointF pixel) => _calibrator.PixelToWorld(pixel);
 
     public Image<Bgr, byte>? CurrentImage => _currentImage;
@@ -138,6 +250,82 @@ public class CalibrationViewModel : BindableBase
 
     private void RaiseEvent(EventHandler? handler) => handler?.Invoke(this, EventArgs.Empty);
 
+    private void OnLiveImageUpdated(object? sender, LiveImageEventArgs e)
+    {
+        if (!_isLiveView) return;
+        System.Windows.Application.Current?.Dispatcher?.BeginInvoke(() =>
+        {
+            ImageSource = e.Image;
+        });
+    }
+
+    /// <summary>
+    /// 绘制检测结果叠加层（轮廓 + 中心十字 + 坐标文字）
+    /// </summary>
+    private void DrawDetectionOverlay(CaptureResult result)
+    {
+        if (result.Image == null) return;
+
+        int w = result.Image.PixelWidth;
+        int h = result.Image.PixelHeight;
+
+        // 创建透明叠加层
+        var visual = new System.Windows.Media.DrawingVisual();
+        using (var dc = visual.RenderOpen())
+        {
+            // 绘制轮廓
+            if (result.ContourPoints != null && result.ContourPoints.Length > 2)
+            {
+                var pen = new System.Windows.Media.Pen(System.Windows.Media.Brushes.Lime, 2);
+                var geometry = new System.Windows.Media.StreamGeometry();
+                using (var ctx = geometry.Open())
+                {
+                    ctx.BeginFigure(new System.Windows.Point(result.ContourPoints[0].X, result.ContourPoints[0].Y), false, true);
+                    for (int i = 1; i < result.ContourPoints.Length; i++)
+                        ctx.LineTo(new System.Windows.Point(result.ContourPoints[i].X, result.ContourPoints[i].Y), true, false);
+                }
+                geometry.Freeze();
+                dc.DrawGeometry(null, pen, geometry);
+            }
+
+            // 绘制中心十字
+            double cx = result.PixelX;
+            double cy = result.PixelY;
+            double crossSize = Math.Max(10, Math.Max(w, h) / 80.0);
+            var crossPen = new System.Windows.Media.Pen(System.Windows.Media.Brushes.Red, 2);
+            dc.DrawLine(crossPen, new System.Windows.Point(cx - crossSize, cy), new System.Windows.Point(cx + crossSize, cy));
+            dc.DrawLine(crossPen, new System.Windows.Point(cx, cy - crossSize), new System.Windows.Point(cx, cy + crossSize));
+
+            // 绘制坐标文字
+            var text = new System.Windows.Media.FormattedText(
+                $"({result.PixelX:F1}, {result.PixelY:F1})",
+                System.Globalization.CultureInfo.CurrentCulture,
+                System.Windows.FlowDirection.LeftToRight,
+                new System.Windows.Media.Typeface("Segoe UI"),
+                Math.Max(12, Math.Max(w, h) / 60.0),
+                System.Windows.Media.Brushes.Yellow,
+                1.0);
+            dc.DrawText(text, new System.Windows.Point(cx + crossSize + 4, cy - text.Height / 2));
+        }
+
+        var rtb = new System.Windows.Media.Imaging.RenderTargetBitmap(w, h, 96, 96, System.Windows.Media.PixelFormats.Pbgra32);
+        rtb.Render(visual);
+        rtb.Freeze();
+        OverlayImage = rtb;
+    }
+
+    /// <summary>
+    /// 停止实时预览并清理资源（窗口关闭时调用）
+    /// </summary>
+    public void Cleanup()
+    {
+        if (_captureService != null)
+        {
+            _captureService.StopLivePreview();
+            _captureService.LiveImageUpdated -= OnLiveImageUpdated;
+        }
+    }
+
     private static BitmapSource MatToBitmapSource(Mat mat)
     {
         using var bitmap = mat.ToBitmap();
diff --git a/XplorePlane/Services/Calibration/NavigationCalibrationCaptureService.cs b/XplorePlane/Services/Calibration/NavigationCalibrationCaptureService.cs
new file mode 100644
index 0000000..e7c9fde
--- /dev/null
+++ b/XplorePlane/Services/Calibration/NavigationCalibrationCaptureService.cs
@@ -0,0 +1,245 @@
+using System;
+using System.Drawing;
+using System.Threading;
+using System.Windows.Media;
+using System.Windows.Media.Imaging;
+using Emgu.CV;
+using Emgu.CV.CvEnum;
+using Emgu.CV.Structure;
+using Emgu.CV.Util;
+using Serilog;
+using XP.Camera;
+using XP.Camera.Calibration;
+using XP.Hardware.MotionControl.Abstractions;
+using XP.Hardware.MotionControl.Abstractions.Enums;
+
+namespace XplorePlane.Services.Calibration;
+
+/// <summary>
+/// 导航相机标定采集服务实现
+/// 读取编码器坐标 + 导航相机拍图 + 图像识别标记中心（亚像素）
+/// </summary>
+public class NavigationCalibrationCaptureService : ICalibrationCaptureService
+{
+    private static readonly ILogger _logger = Log.ForContext<NavigationCalibrationCaptureService>();
+    private readonly IMotionSystem _motionSystem;
+    private readonly ICameraController _navCamera;
+    private BitmapSource? _lastCapturedImage;
+    private readonly object _captureLock = new();
+    private ManualResetEventSlim? _imageReadyEvent;
+    private bool _livePreviewActive;
+
+    public event EventHandler<LiveImageEventArgs>? LiveImageUpdated;
+
+    public NavigationCalibrationCaptureService(IMotionSystem motionSystem, ICameraController navCamera)
+    {
+        _motionSystem = motionSystem;
+        _navCamera = navCamera;
+    }
+
+    public bool IsAvailable => _navCamera.IsConnected;
+
+    public void StartLivePreview()
+    {
+        if (!IsAvailable || _livePreviewActive) return;
+        _livePreviewActive = true;
+        _navCamera.ImageGrabbed += OnLiveImageGrabbed;
+
+        // 如果相机没在采集，启动采集并触发第一帧
+        if (!_navCamera.IsGrabbing)
+        {
+            _navCamera.StartGrabbing();
+            _navCamera.ExecuteSoftwareTrigger();
+        }
+
+        _logger.Information("标定实时预览已启动");
+    }
+
+    public void StopLivePreview()
+    {
+        _livePreviewActive = false;
+        _navCamera.ImageGrabbed -= OnLiveImageGrabbed;
+        _logger.Information("标定实时预览已停止");
+    }
+
+    private void OnLiveImageGrabbed(object? sender, ImageGrabbedEventArgs e)
+    {
+        if (!_livePreviewActive) return;
+        var bmp = ConvertToBitmapSource(e);
+        if (bmp != null)
+        {
+            _lastLiveImage = bmp;
+            LiveImageUpdated?.Invoke(this, new LiveImageEventArgs(bmp));
+        }
+    }
+
+    private volatile BitmapSource? _lastLiveImage;
+
+    public CaptureResult? CaptureCurrentPoint()
+    {
+        if (!IsAvailable)
+        {
+            _logger.Warning("采集失败：导航相机未连接");
+            return null;
+        }
+
+        // 1. 读取编码器坐标
+        var xAxis = _motionSystem.GetLinearAxis(AxisId.StageX);
+        var yAxis = _motionSystem.GetLinearAxis(AxisId.StageY);
+        xAxis.UpdateStatus();
+        yAxis.UpdateStatus();
+        double worldX = xAxis.ActualPosition;
+        double worldY = yAxis.ActualPosition;
+
+        // 2. 导航相机拍图
+        var image = CaptureImage();
+        if (image == null)
+        {
+            _logger.Warning("采集失败：无法获取导航相机图像");
+            return null;
+        }
+
+        // 3. 图像识别标记中心（亚像素）
+        var grayImage = BitmapSourceToGray(image);
+        var detection = DetectMarkerCenter(grayImage);
+        grayImage.Dispose();
+
+        if (detection == null)
+        {
+            _logger.Warning("采集失败：未能识别标记点");
+            return null;
+        }
+
+        _logger.Information("标定点采集成功: Pixel=({Px:F1}, {Py:F1}), World=({Wx:F3}, {Wy:F3})",
+            detection.Value.Center.X, detection.Value.Center.Y, worldX, worldY);
+
+        return new CaptureResult
+        {
+            PixelX = detection.Value.Center.X,
+            PixelY = detection.Value.Center.Y,
+            WorldX = worldX,
+            WorldY = worldY,
+            Image = image,
+            ContourPoints = detection.Value.Contour
+        };
+    }
+
+    public BitmapSource? CaptureImage()
+    {
+        if (!_navCamera.IsConnected) return null;
+
+        // 如果实时预览在运行，直接使用最新一帧
+        if (_livePreviewActive && _lastLiveImage != null)
+            return _lastLiveImage;
+
+        lock (_captureLock)
+        {
+            _lastCapturedImage = null;
+            _imageReadyEvent = new ManualResetEventSlim(false);
+
+            void OnImageGrabbed(object? sender, ImageGrabbedEventArgs e)
+            {
+                _lastCapturedImage = ConvertToBitmapSource(e);
+                _imageReadyEvent?.Set();
+            }
+
+            _navCamera.ImageGrabbed += OnImageGrabbed;
+
+            try
+            {
+                if (!_navCamera.IsGrabbing)
+                    _navCamera.StartGrabbing();
+                _navCamera.ExecuteSoftwareTrigger();
+
+                // 等待图像到达（超时 3 秒）
+                if (!_imageReadyEvent.Wait(3000))
+                {
+                    _logger.Warning("导航相机采集超时");
+                    return null;
+                }
+
+                return _lastCapturedImage;
+            }
+            finally
+            {
+                _navCamera.ImageGrabbed -= OnImageGrabbed;
+                _imageReadyEvent?.Dispose();
+                _imageReadyEvent = null;
+            }
+        }
+    }
+
+    /// <summary>
+    /// 检测图像中标记点的中心（亚像素精度）
+    /// 支持圆点标记：阈值分割 → 轮廓检测 → 面积过滤 → 亚像素质心
+    /// </summary>
+    private (PointF Center, Point[] Contour)? DetectMarkerCenter(Image<Gray, byte> grayImage)
+    {
+        int w = grayImage.Width, h = grayImage.Height;
+
+        // 高斯模糊降噪
+        using var blurred = new Image<Gray, byte>(w, h);
+        CvInvoke.GaussianBlur(grayImage, blurred, new Size(5, 5), 1.0);
+
+        // Otsu 自动阈值二值化
+        using var binary = new Image<Gray, byte>(w, h);
+        CvInvoke.Threshold(blurred, binary, 0, 255, ThresholdType.Otsu | ThresholdType.Binary);
+
+        // 轮廓检测
+        using var contours = new VectorOfVectorOfPoint();
+        using var hierarchy = new Mat();
+        CvInvoke.FindContours(binary, contours, hierarchy, RetrType.External, ChainApproxMethod.ChainApproxSimple);
+
+        // 找到面积最大的轮廓（假设标记是视野中最显著的特征）
+        double maxArea = 0;
+        int bestIdx = -1;
+        double minValidArea = w * h * 0.001;  // 最小有效面积：图像面积的 0.1%
+        double maxValidArea = w * h * 0.5;    // 最大有效面积：图像面积的 50%
+
+        for (int i = 0; i < contours.Size; i++)
+        {
+            double area = CvInvoke.ContourArea(contours[i]);
+            if (area < minValidArea || area > maxValidArea) continue;
+            if (area > maxArea)
+            {
+                maxArea = area;
+                bestIdx = i;
+            }
+        }
+
+        if (bestIdx < 0) return null;
+
+        // 亚像素质心计算
+        var moments = CvInvoke.Moments(contours[bestIdx]);
+        if (moments.M00 < 1) return null;
+
+        double cx = moments.M10 / moments.M00;
+        double cy = moments.M01 / moments.M00;
+
+        var contourPoints = contours[bestIdx].ToArray();
+        return (new PointF((float)cx, (float)cy), contourPoints);
+    }
+
+    private static Image<Gray, byte> BitmapSourceToGray(BitmapSource bmp)
+    {
+        var converted = new FormatConvertedBitmap(bmp, PixelFormats.Bgra32, null, 0);
+        int w = converted.PixelWidth, h = converted.PixelHeight;
+        int stride = w * 4;
+        var pixels = new byte[stride * h];
+        converted.CopyPixels(pixels, stride, 0);
+        var gray = new Image<Gray, byte>(w, h);
+        for (int y = 0; y < h; y++)
+            for (int x = 0; x < w; x++)
+            {
+                int idx = y * stride + x * 4;
+                gray.Data[y, x, 0] = (byte)(pixels[idx + 2] * 0.299 + pixels[idx + 1] * 0.587 + pixels[idx] * 0.114);
+            }
+        return gray;
+    }
+
+    private static BitmapSource? ConvertToBitmapSource(ImageGrabbedEventArgs e)
+    {
+        if (e.Width <= 0 || e.Height <= 0 || e.PixelData == null) return null;
+        return PixelConverter.ToBitmapSource(e.PixelData, e.Width, e.Height, e.PixelFormat);
+    }
+}
diff --git a/XplorePlane/ViewModels/Main/MainViewModel.cs b/XplorePlane/ViewModels/Main/MainViewModel.cs
index cb537a3..a653b38 100644
--- a/XplorePlane/ViewModels/Main/MainViewModel.cs
+++ b/XplorePlane/ViewModels/Main/MainViewModel.cs
@@ -676,14 +676,28 @@ namespace XplorePlane.ViewModels
             };
 
             var calibrationDialogService = new XP.Camera.Calibration.DefaultCalibrationDialogService();
-            var calibrationViewModel = new XP.Camera.Calibration.ViewModels.CalibrationViewModel(calibrationDialogService);
+
+            // 尝试创建采集服务（需要运动系统和导航相机）
+            XP.Camera.Calibration.ICalibrationCaptureService? captureService = null;
+            try
+            {
+                var motionSystem = Prism.Ioc.ContainerLocator.Current?.Resolve<XP.Hardware.MotionControl.Abstractions.IMotionSystem>();
+                var navCamera = Prism.Ioc.ContainerLocator.Current?.Resolve<XP.Camera.ICameraController>();
+                if (motionSystem != null && navCamera != null)
+                    captureService = new Services.Calibration.NavigationCalibrationCaptureService(motionSystem, navCamera);
+            }
+            catch { /* 采集服务不可用时降级为手动模式 */ }
+
+            var calibrationViewModel = new XP.Camera.Calibration.ViewModels.CalibrationViewModel(calibrationDialogService, captureService);
             var calibrationControl = new XP.Camera.Calibration.Controls.CalibrationControl
             {
                 DataContext = calibrationViewModel
             };
 
             calibrationWindow.Content = calibrationControl;
-            calibrationWindow.ShowDialog();
+            calibrationWindow.Closed += (s, e) => calibrationViewModel.Cleanup();
+            calibrationWindow.Owner = System.Windows.Application.Current.MainWindow;
+            calibrationWindow.Show();
         }
 
         private void ExecuteOpenSettings()